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endothelial cell growth media 2  (PromoCell)


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    Structured Review

    PromoCell endothelial cell growth media 2
    Endothelial Cell Growth Media 2, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endothelial+growth+media/Endothelial+Cell+Growth+Medium+2+SupplementPack/bio_rxiv__64898__2026__05__20__726520-62-50-55
    Average 97 stars, based on 195 article reviews
    endothelial cell growth media 2 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Genomic profiling informs diagnoses and treatment in vascular anomalies
    Article Snippet: .. Cells were collected from lymphatic fluid samples by centrifugation (200× g , 10 min), resuspended in complete endothelial growth media (ECGM-MV2, Promocell) supplemented with 20% fetal bovine serum (FBS, VWR) and plated on one fibronectin coated plate. ..

    Article Title: Genomic profiling informs diagnoses and treatment in vascular anomalies.
    Article Snippet: Dong Li , Sarah E. Sheppard , Michael E. March, Mark R. Battig, Lea F. Surrey , Abhay S. Srinivasan , Leticia S. Matsuoka, Lifeng Tian, Fengxiang Wang, Christoph Seiler, Jill Dayneka , Alexandra J. Borst , Mary C. Matos, Scott M. Paulissen , Ganesh Krishnamurthy, Bede Nriagu , Tamjeed Sikder, Melissa Casey, Lydia Williams , Sneha Rangu, Nora O’Connor, Alexandria Thomas, Erin Pinto, Cuiping Hou, Kenny Nguyen, Renata Pellegrino da Silva, Samar N. Chehimi, Charlly Kao, Lauren Biroc, Allison D. Britt , Maria Queenan, Janet R. Reid , Joseph A. Napoli, David M. Low, Seth Vatsky, James Treat, Christopher L. Smith, Anne Marie Cahill, Kristen M. Snyder, Denise M. Adams, Yoav Dori & Hakon Hakonarson

    Cell Culture:

    Article Title: Inhibition of DAPK3 Suppresses Radiation-Induced Cellular Senescence by Activation of a PGC1α-Dependent Metabolism Pathway in Brain Endothelial Cells.
    Article Snippet: In the brain, environmental changes, such as neuroinflammation, can induce senescence, characterized by the decreased proliferation of neurons and dendrites and synaptic and vascular damage, resulting in cognitive decline.. Senescence promotes neuroinflammatory disorders by senescence-associated secretory phenotypes and reactive oxygen species.. In human brain microvascular endothelial cells (HBMVECs), we demonstrate that chronological aging and irradiation increase death-associated protein kinase 3 (DAPK3) expression.

    Incubation:

    Article Title: Inhibition of DAPK3 Suppresses Radiation-Induced Cellular Senescence by Activation of a PGC1α-Dependent Metabolism Pathway in Brain Endothelial Cells.
    Article Snippet: In the brain, environmental changes, such as neuroinflammation, can induce senescence, characterized by the decreased proliferation of neurons and dendrites and synaptic and vascular damage, resulting in cognitive decline.. Senescence promotes neuroinflammatory disorders by senescence-associated secretory phenotypes and reactive oxygen species.. In human brain microvascular endothelial cells (HBMVECs), we demonstrate that chronological aging and irradiation increase death-associated protein kinase 3 (DAPK3) expression.

    Article Title: Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness
    Article Snippet: .. To measure in vitro angiogenesis, 48‐well plates were coated with 60 μL of growth factor reduced Matrigel (Corning; 356230) and incubated at 37°C for 30 min. P4 HUVECs were then seeded at 35,000 cells/well with either endothelial growth media (PromoCell; C‐22121) with 1% penicillin–streptomycin (positive control), endothelial basal media (negative control), or endothelial basal media (PromoCell; C‐22221) with 0.1% FBS and 1% penicillin–streptomycin with 5E9 EVs/mL. .. 3–6 h later, tubes were imaged using a Nikon Eclipse Ti2 Microscope at 2× magnification and the number of fully formed loops were counted.

    Article Title: Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness
    Article Snippet: .. To measure in vitro angiogenesis, 48-well plates were coated with 60 μL of growth factor reduced Matrigel (Corning; 356230) and incubated at 37 C for 30 min. P4 HUVECs were then seeded at 35,000 cells/well with either endothelial growth media (PromoCell; C-22121) with 1% penicillin–streptomycin (positive control), endothelial basal media (negative control), or endothelial basal media (PromoCell; C-22221) with 0.1% FBS and 1% penicillin–streptomycin with 5E9 EVs/mL. .. 3–6 h later, tubes were imaged using a Nikon Eclipse Ti2 Microscope at 2 magnification and the number of fully formed loops were counted.

    In Vitro:

    Article Title: Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness
    Article Snippet: .. To measure in vitro angiogenesis, 48‐well plates were coated with 60 μL of growth factor reduced Matrigel (Corning; 356230) and incubated at 37°C for 30 min. P4 HUVECs were then seeded at 35,000 cells/well with either endothelial growth media (PromoCell; C‐22121) with 1% penicillin–streptomycin (positive control), endothelial basal media (negative control), or endothelial basal media (PromoCell; C‐22221) with 0.1% FBS and 1% penicillin–streptomycin with 5E9 EVs/mL. .. 3–6 h later, tubes were imaged using a Nikon Eclipse Ti2 Microscope at 2× magnification and the number of fully formed loops were counted.

    Article Title: Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness
    Article Snippet: .. To measure in vitro angiogenesis, 48-well plates were coated with 60 μL of growth factor reduced Matrigel (Corning; 356230) and incubated at 37 C for 30 min. P4 HUVECs were then seeded at 35,000 cells/well with either endothelial growth media (PromoCell; C-22121) with 1% penicillin–streptomycin (positive control), endothelial basal media (negative control), or endothelial basal media (PromoCell; C-22221) with 0.1% FBS and 1% penicillin–streptomycin with 5E9 EVs/mL. .. 3–6 h later, tubes were imaged using a Nikon Eclipse Ti2 Microscope at 2 magnification and the number of fully formed loops were counted.

    Positive Control:

    Article Title: Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness
    Article Snippet: .. To measure in vitro angiogenesis, 48‐well plates were coated with 60 μL of growth factor reduced Matrigel (Corning; 356230) and incubated at 37°C for 30 min. P4 HUVECs were then seeded at 35,000 cells/well with either endothelial growth media (PromoCell; C‐22121) with 1% penicillin–streptomycin (positive control), endothelial basal media (negative control), or endothelial basal media (PromoCell; C‐22221) with 0.1% FBS and 1% penicillin–streptomycin with 5E9 EVs/mL. .. 3–6 h later, tubes were imaged using a Nikon Eclipse Ti2 Microscope at 2× magnification and the number of fully formed loops were counted.

    Article Title: Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness
    Article Snippet: .. To measure in vitro angiogenesis, 48-well plates were coated with 60 μL of growth factor reduced Matrigel (Corning; 356230) and incubated at 37 C for 30 min. P4 HUVECs were then seeded at 35,000 cells/well with either endothelial growth media (PromoCell; C-22121) with 1% penicillin–streptomycin (positive control), endothelial basal media (negative control), or endothelial basal media (PromoCell; C-22221) with 0.1% FBS and 1% penicillin–streptomycin with 5E9 EVs/mL. .. 3–6 h later, tubes were imaged using a Nikon Eclipse Ti2 Microscope at 2 magnification and the number of fully formed loops were counted.

    Negative Control:

    Article Title: Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness
    Article Snippet: .. To measure in vitro angiogenesis, 48‐well plates were coated with 60 μL of growth factor reduced Matrigel (Corning; 356230) and incubated at 37°C for 30 min. P4 HUVECs were then seeded at 35,000 cells/well with either endothelial growth media (PromoCell; C‐22121) with 1% penicillin–streptomycin (positive control), endothelial basal media (negative control), or endothelial basal media (PromoCell; C‐22221) with 0.1% FBS and 1% penicillin–streptomycin with 5E9 EVs/mL. .. 3–6 h later, tubes were imaged using a Nikon Eclipse Ti2 Microscope at 2× magnification and the number of fully formed loops were counted.

    Article Title: Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness
    Article Snippet: .. To measure in vitro angiogenesis, 48-well plates were coated with 60 μL of growth factor reduced Matrigel (Corning; 356230) and incubated at 37 C for 30 min. P4 HUVECs were then seeded at 35,000 cells/well with either endothelial growth media (PromoCell; C-22121) with 1% penicillin–streptomycin (positive control), endothelial basal media (negative control), or endothelial basal media (PromoCell; C-22221) with 0.1% FBS and 1% penicillin–streptomycin with 5E9 EVs/mL. .. 3–6 h later, tubes were imaged using a Nikon Eclipse Ti2 Microscope at 2 magnification and the number of fully formed loops were counted.



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    Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for <t>endothelial</t> cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.
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    Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for <t>endothelial</t> cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.
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    Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for <t>endothelial</t> cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.
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    Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for endothelial cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.

    Journal: Bioactive Materials

    Article Title: Ribbon-shaped microgels as bioinks for 3D bioprinting of anisotropic tissue structures

    doi: 10.1016/j.bioactmat.2025.12.040

    Figure Lengend Snippet: Effect of extrusion process on μRB bioink and cell alignment. (A) Schematic of MSCs encapsulated in μRB bioink with HUVECs seeded on top of the printed scaffolds. (B) Live cell staining of MSC alignment on individual μRBs (Scale bar = 100 μm). (C) Distribution of MSC cell length (n = 250 per group); p-values were determined by one-way analysis of variance (ANOVA) with Tukey's multiple comparisons test; ∗∗∗∗p < 0.0001. (D) Cell orientation relative to μRB orientation, where 0° is parallel to the axis of the μRB (n = 250 per group). (E) Confocal images of F-Actin staining for cell morphology and VE-cadherin staining for endothelial cell junctions. Color survey visualization of directional analysis conducted using OrientationJ (Scale bar = 200 μm). (F, G) Quantification of F-actin and VE-Cadherin alignment. For alignment quantification (n = 10 per group) data reported as mean ± S.D., statistical analysis by Watson–Wheeler test, ∗p ≤ 0.05.

    Article Snippet: Human umbilical vein endothelial cells (HUVECs, Lonza) were cultured in Endothelial Cell Growth Media (R&D Systems).

    Techniques: Staining